Non-invasive detection of universal osteodifferentiation signatures through untargeted NMR Exometabolomics

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abstract

The secretion profile of mesenchymal stem cells (MSCs) is a major driver of their therapeutic effects, although secretome metabolites are largely unexplored. Exometabolomics may reveal new information on MSCs, providing means to monitor osteodifferentiation. Here, untargeted NMR metabolomics was applied to monitor exometabolome of human adipose-derived MSCs from 3 different donors, throughout 21 days of osteodifferentiation. Results revealed changes in metabolic activity, including energy metabolic switch, cell membrane metabolism and antioxidative protection. The donor-independent features may be exploited to assess cell osteogenic ability and advance metabolic candidates for media optimization.

authors

Bispo, DSC; Rodrigues, JA; Oliveira, MB; Mano, JF; Gil, AM

Groups

Projects

Metabolite-activated 3D stem cell differentiation into bone (BetterBone)

A Metabolomics-guided Bioreactor for Improved Engineered Bone Implants (BioImplant)

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

acknowledgements

BetterBone (2022.04286.PTDC, DOI 10.54499/2022.04286.PTDC) & BIOIMPLANT (PTDC/BTM-ORG/28835/2017), through COMPETE2020/FEDER (POCI-01-0145-FEDER-028835). CICECO-Institute of Materials Aveiro, UIDB/50011/2020 (DOI 10.54499/UIDB/50011/2020), UIDP/50011/2020 (DOI 10.54499/UIDP/50011/2020) & LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020), financed by national funds through the FCT/MCTES (PIDDAC). SFRH/BD/150655/2020 (DOI 10.54499/SFRH/BD/150655/2020, DSB PhD grant). National NMR Network (Project Nº022161) through FEDER (COMPETE 2020/POCI/PORL/FCT through PIDDAC).

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Publication Details

type
Conference Communication
year
2024
link
https://app.encontrociencia.pt/exhibitor/22929

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