Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
authors Vicente, FA; Lario, LD; Pessoa, A; Ventura, SPM
nationality International
journal PROCESS BIOCHEMISTRY
author keywords Bromelain; Aqueous micellar two-phase system; Triton X-114; Ionic liquids; Co-surfactant; Purification
keywords COMOSUS L. MERRYL; ANANAS-COMOSUS; EXTRACT BROMELAIN; REVERSED MICELLES; BIPHASIC SYSTEMS; PEEL WASTE; PURIFICATION; OPTIMIZATION; SEPARATION; PROTEINS
abstract Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1359-5113
year published 2016
volume 51
issue 4
beginning page 528
ending page 534
digital object identifier (doi) 10.1016/j.procbio.2016.01.004
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
unique article identifier WOS:000373419700008
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