abstract
Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved.
keywords
COMOSUS L. MERRYL; ANANAS-COMOSUS; EXTRACT BROMELAIN; REVERSED MICELLES; BIPHASIC SYSTEMS; PEEL WASTE; PURIFICATION; OPTIMIZATION; SEPARATION; PROTEINS
subject category
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
authors
Vicente, FA; Lario, LD; Pessoa, A; Ventura, SPM
our authors
Filipa Alexandra André Vicente
PhD Student
Sónia Patrícia Marques Ventura
Assistant Professoracknowledgements
The authors are grateful for financial support from FEDER funds through the program COMPETE and by national fund through the Portuguese Foundation for Science and Technology (FCT) under the scope of the project Pest-C/CTM/LA0011/2013, and the doctoral and post-doctoral grants SFRH/BD/101683/2014 and SFRH/BPD/79263/2011 of F.A. Vicente and S.P.M. Ventura, respectively. The authors acknowledge the financial support from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) by the PhD fellowship process no. 2012/23726-4 awarded to Luciana Lario and the project FAPESP 2013\08617-7.