abstract
Pinus elliottii var. elliottii is a pine species with enormous economic value particularly for timber and resin industries, and is subject of high pressure for genetic improvement and cloning elite genotypes. We have recently developed a robust micropropagation protocol for this species. Plantlets performance needs to be evaluated to validate this protocol for further mass propagation. Micropropagated plantlets and seed derived plants with similar age and shoot length were compared regarding photosynthesis, carbohydrates and pigments content, water status, DNA content and cell cycle dynamics. Micropropagated plantlets had an overall physiological performance similar to seed-derived plants. In particular, except for the transpiration rate (E), CO2 assimilation rate (A) and total soluble sugars (TSS) content, no major differences between plantlets and seedlings in terms of relative water content (RWC), chlorophyll a fluorescence and pigments content were found. Genetic fidelity analyses support that the micro propagation protocol neither induce DNA content changes nor alterations in cell cycle dynamics. (C) 2016 Elsevier Masson SAS. All rights reserved.
keywords
DNA FLOW-CYTOMETRY; GENOME SIZE; PHOTOSYNTHETIC PARAMETERS; PLANTLET REGENERATION; GENETIC STABILITY; ACCLIMATIZATION; MICROPROPAGATION; PERFORMANCE; MARKERS; LEAVES
subject category
Plant Sciences
authors
Nunes, S; Santos, C; Moutinho-Pereira, J; Correia, C; Oliveira, H; de Oliveira, JMF; Pereira, VT; Almeida, T; Marum, L; Dias, MC
our authors
Helena Oliveira
Post-Doc FellowshipGroups
acknowledgements
This research was supported by the project CENTRO-07-0202-FEDER-018579-