Physiological characterization and true-to-typeness evaluation of in vitro and ex vitro seedlings of Pinus elliottii: A contribution to breeding programs
authors Nunes, S; Santos, C; Moutinho-Pereira, J; Correia, C; Oliveira, H; de Oliveira, JMF; Pereira, VT; Almeida, T; Marum, L; Dias, MC
nationality International
journal PLANT PHYSIOLOGY AND BIOCHEMISTRY
author keywords Genetic stability; Micropropagation; Photosynthesis; Slash pine
keywords DNA FLOW-CYTOMETRY; GENOME SIZE; PHOTOSYNTHETIC PARAMETERS; PLANTLET REGENERATION; GENETIC STABILITY; ACCLIMATIZATION; MICROPROPAGATION; PERFORMANCE; MARKERS; LEAVES
abstract Pinus elliottii var. elliottii is a pine species with enormous economic value particularly for timber and resin industries, and is subject of high pressure for genetic improvement and cloning elite genotypes. We have recently developed a robust micropropagation protocol for this species. Plantlets performance needs to be evaluated to validate this protocol for further mass propagation. Micropropagated plantlets and seed derived plants with similar age and shoot length were compared regarding photosynthesis, carbohydrates and pigments content, water status, DNA content and cell cycle dynamics. Micropropagated plantlets had an overall physiological performance similar to seed-derived plants. In particular, except for the transpiration rate (E), CO2 assimilation rate (A) and total soluble sugars (TSS) content, no major differences between plantlets and seedlings in terms of relative water content (RWC), chlorophyll a fluorescence and pigments content were found. Genetic fidelity analyses support that the micro propagation protocol neither induce DNA content changes nor alterations in cell cycle dynamics. (C) 2016 Elsevier Masson SAS. All rights reserved.
publisher ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
issn 0981-9428
year published 2016
volume 107
beginning page 222
ending page 227
digital object identifier (doi) 10.1016/j.plaphy.2016.05.039
web of science category Plant Sciences
subject category Plant Sciences
unique article identifier WOS:000382341600022
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journal impact factor 3.72
5 year journal impact factor 3.966
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