abstract
Exogenous administration of nitric oxide may be a therapy for several pathologies because this molecule regulates many biological systems. Here, the storage and release of NO by microporous titanosilicate ETS-10 and samples where the silicon was substituted by aluminium (ETAS-10) or gallium (ETGS-10) are studied. The Al- and Ga-doped materials exhibit an increase in the storage capacity of 95% and 55%, respectively, the highest values observed, so far, for microporous titanosilicates. ETAS-10 releases more NO and ETGS-10 almost the same amount as ETS-10. In ETAS-10 and ETGS-10, the irreversibly adsorbed NO amount increases relatively to ETS-10. Tests of NO release in haemoglobin solutions indicate that biologically relevant amounts are release and that ETS-10 and ETGS-10 display a release slower than ETAS-10, more adequate for a sustained delivery. Cytotoxicity studies show that the samples have very low toxicity (cell viability above 87%, after 72 h) at high concentration (0.45 mg cm(-3)). Tests at variable ETS-10 concentration further confirm the low cytotoxicity of this material, even at high concentrations (up to 1.8 mg cm(-3)). (C) 2016 Elsevier Inc. All rights reserved.
keywords
HELA CANCER-CELLS; DRUG-DELIVERY; SLOW-RELEASE; ZEOLITE STRUCTURES; SURFACE-PROPERTIES; NANOPARTICLES; CYTOTOXICITY; ADSORPTION; NO; AVOIDANCE
subject category
Chemistry; Science & Technology - Other Topics; Materials Science
authors
Pinto, ML; Fernandes, AC; Antunes, F; Pires, J; Rocha, J
our authors
João Rocha
Full ProfessorProjects
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
Nanoporous materials as multiple therapeutic agents (IF/00993/2012/CP0172/CT0013)
acknowledgements
This work was supported by Fundacao para a Ciencia e a Tecnologia to CICECO - Aveiro Institute of Materials (POCI-01-8070145-FEDER-007679 vertical bar UID/CTM/50011/2013, CERENA (UID/ECI/04028/2013) and CQB (UID/MULTI/00612/2013) research centers and project IF/00993/2012/CP0172/CT0013, financed by national funds through the FCT/MEC and when applicable co-financed by FEDER and COMPETE under the PT2020 Partnership Agreement. ACF gratefully thanks FCT for the doctoral fellowship (SFRH/BD/72058/2010) and MPL thanks for the Investigador FCT contract (IF/00993/2012). The authors thank Zhi Lin for the some of the samples tested.