abstract
Background: Aescin, a natural mixture of saponins occurring in Aesculus hippocastanum, exhibits important flebotonic properties, being used in the treatment of chronic venous insufficiency in legs. The inclusion of aescin into cyclodextrins (CDs) is a technical solution for its incorporation into the textile of stockings, but details of the physicochemistry of these host-guest systems are lacking. This work investigates the inclusion of aescin into the cavities of two native cyclodextrins, beta-CD and gamma-CD. Results: The continuous variation method applied to aqueous-phase H-1 nuclear magnetic resonance (H-1 NMR) has demonstrated that the preferred CD/aescin inclusion stoichiometries are 2:1 with beta-CD and 1:1 with gamma-CD. The affinity constant calculated for gamma-CD center dot aescin was 894 M-1, while for 2 beta-CD center dot aescin it was estimated to be 715 M-1. Density functional theory (DFT) calculations on the interaction of aescin Ib with CDs show that an inclusion can indeed occur and it is further demonstrated that the wider cavity of gamma-CD is more adequate to accommodate this large guest. ROESY spectroscopy is consistent with the formation of a complex in which the triterpenic moiety of aescin is included into the cavity of gamma-CD. The higher stability of this geometry was confirmed by DFT. Furthermore, DFT calculations were applied to determine the chemical shifts of the protons H3 and H5 of the CDs in the optimised structures of the inclusion complexes. The calculated values are very similar to the experimental data, validating the approach made in this study by NMR. Conclusion: The combination of experimental data from aqueous-state NMR measurements and theoretical calculations has demonstrated that gamma-CD is the most suitable host for aescin, although the inclusion also occurs with beta-CD. The geometry of the gamma-CD center dot aescin complex is characterised by the inclusion of the triterpene segment of aescin into the host cavity.
keywords
CONSISTENT PERTURBATION-THEORY; IN-VITRO; BETULINIC ACID; COMPLEX; PROLIFERATION; STATES; SWEET; CELLS; WATER; MICE
subject category
Science & Technology - Other Topics; Materials Science; Physics
authors
Ramos, AI; Vaz, PD; Braga, SS; Silva, AMS
our authors
Pedro Miguel Duarte Vaz
Collaborator
acknowledgements
The authors acknowledge the University of Aveiro and FCT/MEC (Fundacao para a Ciencia e a Tecnologia, Ministerio da Educacao e da Ciencia), through national funds and, where applicable, co-financed by the FEDER (European Fund for Regional Development) within the PT2020 Partnership Agreement, for the financial support to the QOPNA research project (FCT UID/QUI/00062/2013) and the Portuguese NMR Network. DFT calculations were made possible due to the computing resources provided by STFC Scientific Computing Department's SCARF cluster.