Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells
authors Srikanth, K; Trindade, T; Duarte, AC; Pereira, E
nationality International
journal ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
author keywords Chinook salmon; Iron oxide nanoparticles; Cytotoxicity; Oxidative stress; Lipid peroxidation; Protein carbonyl
keywords EPITHELIAL-CELLS; IN-VITRO; GLUTATHIONE; TOXICITY; FISH; LINES; ASSAY; ACID; GENOTOXICITY; INDUCTION
abstract The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4 NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4 NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 mu g/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 mu g/ml concentration of Fe3O4 NPs after 24 h. Fe3O4 NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 mu g/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe3O4 NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.
publisher SPRINGER HEIDELBERG
issn 0944-1344
isbn 1614-7499
year published 2017
volume 24
issue 2
beginning page 2055
ending page 2064
digital object identifier (doi) 10.1007/s11356-016-7870-z
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
unique article identifier WOS:000394254000088
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journal impact factor 3.056
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