MC3T3-E1 pre-osteoblast response and differentiation after graphene oxide nanosheet uptake


Nano-graphene oxide (GO) and its functionalized derivatives have aroused a great interest for drug delivery, tissue engineering and photothermal cancer therapy, but their biocompatibility has not yet been fully assessed. The aim of the present study was to evaluate the proliferation and differentiation of MC3T3-E1 pre-osteoblasts after the uptake of GO nanosheets (c.a. 400 nm), functionalized with poly( ethylene glycol amine) (PEG) and labelled with fluorescein isothiocyanate (FITC). Significant proliferation decrease and apoptosis increase were observed 3 days after incorporation of FITC-PEG-GO by MC3T3-E1 cells. However, alterations,on healthy pre-osteoblast differentiation into cells exhibiting osteoblast phenotype were not observed, as they showed normal alkaline phosphatase levels and matrix mineralization 12 days after nanosheet uptake. The results suggest that 40 mu g/mL concentrations of these nanosheets would not affect the differentiation of healthy pre-osteoblasts, thus these PEG-GO nanosheets have potential to be used for biomedical applications after their internalization, as the induction of local hyperthermia on bone cancer. (C) 2017 Elsevier B.V. All rights reserved.



subject category

Biophysics; Chemistry; Materials Science


Cicuendez, M; Silva, VS; Hortiguela, MJ; Matesanz, MC; Vila, M; Portoles, MT

our authors



This study was supported by research grants from the Spanish Ministerio de Economia y Competitividad (MAT2013-43299-R and MAT2016-75611-R AEI/FEDER, UE). M.C. acknowledges the financial support from the FCT [SFRH/BPD/101468/2014 Post-Doctoral grant]. V.S.S. acknowledges financial support from the FCT [SFRH/BPD/110269/2015 Post-Doctoral grant]. M.J.H. acknowledges UID/EMS/00481/2013 Project. The authors wish to thank also to the staff of the Centro de Citometria y Microscopia de Fluorescencia of the Universidad Complutense de Madrid (Spain) for the assistance in the flow cytometry studies, Centro de Espectroscopia y Correlacion of the Universidad Complutense de Madrid (Spain) for DLS studies and ICTS Centro Nacional de Microsocopia Electronica (Spain) for AFM measurements.

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