Immobilization of commercial laccase on spent grain
authors da Silva, AM; Tavares, APM; Rocha, CMR; Cristovao, RO; Teixeira, JA; Macedo, EA
nationality International
journal PROCESS BIOCHEMISTRY
author keywords Brewing; Commercial laccase; Immobilized enzyme; Kinetic parameter; Optimization; Spent grain
keywords TEXTILE DYES DEGRADATION; COVALENT IMMOBILIZATION; PACKED-BED; VERSICOLOR; CHITOSAN; REMOVAL; REACTOR; ENZYME; WATER
abstract aim of this work was to assess the possibility of using beer spent grain (a byproduct of beer's brewing industry) as a carrier for laccase immobilization. Both adsorption (on spent grain - SG and on digested spent grain - DSG)and covalent binding (using glycidol and glyciclol followed by ethylenediamine on DSG) were used. The effect of different immobilization conditions on the immobilization yields and recovered activities such as contact time, enzyme concentration and pH was evaluated. For the best conditions, immobilization yields, recovered activities and thermal, operational and storage stabilities were also evaluated. Finally, the Michaelis-Menten mechanism was applied and the parameter with respect to ABTS oxidation was determined. Enzyme immobilization on DSG led to the best enzyme activities (recovered activities as high as 90%) and to high storage and operational stabilities (10 cycles). Thermal stability was also improved and the half-life of immobilized laccase in SG increased from 0.64h to 1.1 h at 70 degrees C. (c) 2012 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1359-5113
year published 2012
volume 47
issue 7
beginning page 1095
ending page 1101
digital object identifier (doi) 10.1016/j.procbio.2012.03.021
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
unique article identifier WOS:000309333600011
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