Application of statistical experimental methodology to optimize reactive dye decolourization by commercial laccase
authors Tavares, APM; Cristovao, RO; Loureiro, JM; Boaventura, RAR; Macedo, EA
nationality International
journal JOURNAL OF HAZARDOUS MATERIALS
author keywords Biocatalysis; Optimization; Wastewater treatment; Commercial laccase; Reactive dyes
keywords RESPONSE-SURFACE METHODOLOGY; TRAMETES-VERSICOLOR; EXPERIMENTAL-DESIGN; TEXTILE DYES; WASTE-WATER; KRAFT PULP; DECOLORIZATION; DEGRADATION; FACTORIAL; ENZYMES
abstract Three-level Box-Behnken factorial design with three factors (pH, temperature and enzyme concentration) combined with response surface methodology (RSM) was applied to optimize the dye degradation of reactive red 239 (RR239), reactive yellow 15 (RY15) and reactive blue 114 (RB114) dyes by commercial laccase. Mathematical models were developed for each dye showing the effect of each factor and their interactions on colour removal. The model predicted for RY15 that a decolourization above 90% (after 24 h) could be obtained when the enzyme concentration, temperature and pH were set at 109.8 U/L, 39.2 degrees C and 6.6, respectively; whilst for RB114 and RR239 the temperature and enzyme concentration did not affect the decolourization (>90%) in the considered range and optimum pH value was found at 5.5-7.0 and 7.0-7.5, respectively. These predicted values were also experimentally validated. Average final values of responses were in good agreement with calculated values, thus confirming the reliability of the models of RY15, RB114 and RR239 decolourization. (C) 2008 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0304-3894
year published 2009
volume 162
issue 2-3
beginning page 1255
ending page 1260
digital object identifier (doi) 10.1016/j.jhazmat.2008.06.014
web of science category Engineering, Environmental; Environmental Sciences
subject category Engineering; Environmental Sciences & Ecology
unique article identifier WOS:000263370200093
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