Selection and optimization of culture medium for exopolysaccharide production by Coriolus (Trametes) versicolor
authors Tavares, APM; Agapito, MSM; Coelho, MAZ; da Silva, JAL; Barros-Timmons, A; Coutinho, JAP; Xavier, AMRB
nationality International
journal WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
author keywords Coriolus versicolor; exopolysaccharide production; experimental design; optimization; submerged culture
keywords EXO-BIOPOLYMER PRODUCTION; PAECILOMYCES-JAPONICA; CORDYCEPS-SINENSIS; SUBMERGED CULTURE; BROTH RHEOLOGY; POLYSACCHARIDES; MORPHOLOGY; MYCELIUM; SUGAR; FUNGI
abstract Coriolus versicolor is a medicinal fungus producing exopolysaccharides (EPS). Five well-defined culture media were studied to select the medium that maximizes production of EPS by C. versicolor. Biomass, reducing sugars and EPS concentrations along with the rheological behaviour of the broth were followed during fermentations lasting 9 days. The yeast malt extract medium (YM) was shown to yield the highest production of EPS. Fermentation conditions with YM medium were further investigated to optimize EPS production by C. versicolor. An experimental design to do this was adopted, in which the effects of pH and initial substrate concentration were considered. The effects of initial glucose concentration (5, 15 and 25 g l(-1)) and pH (4.0, 5.5 and 7.0) were evaluated. The initial glucose concentration was found to be the most important factor in EPS production and also cell growth.
publisher SPRINGER
issn 0959-3993
year published 2005
volume 21
issue 8-9
beginning page 1499
ending page 1507
digital object identifier (doi) 10.1007/s11274-005-7370-7
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000234411400028
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journal impact factor 2.477
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