Selection and optimization of culture medium for exopolysaccharide production by Coriolus (Trametes) versicolor

authors	Tavares, APM; Agapito, MSM; Coelho, MAZ; da Silva, JAL; Barros-Timmons, A; Coutinho, JAP; Xavier, AMRB
nationality	International
journal	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
author keywords	Coriolus versicolor; exopolysaccharide production; experimental design; optimization; submerged culture
keywords	EXO-BIOPOLYMER PRODUCTION; PAECILOMYCES-JAPONICA; CORDYCEPS-SINENSIS; SUBMERGED CULTURE; BROTH RHEOLOGY; POLYSACCHARIDES; MORPHOLOGY; MYCELIUM; SUGAR; FUNGI
abstract	Coriolus versicolor is a medicinal fungus producing exopolysaccharides (EPS). Five well-defined culture media were studied to select the medium that maximizes production of EPS by C. versicolor. Biomass, reducing sugars and EPS concentrations along with the rheological behaviour of the broth were followed during fermentations lasting 9 days. The yeast malt extract medium (YM) was shown to yield the highest production of EPS. Fermentation conditions with YM medium were further investigated to optimize EPS production by C. versicolor. An experimental design to do this was adopted, in which the effects of pH and initial substrate concentration were considered. The effects of initial glucose concentration (5, 15 and 25 g l(-1)) and pH (4.0, 5.5 and 7.0) were evaluated. The initial glucose concentration was found to be the most important factor in EPS production and also cell growth.
publisher	SPRINGER
issn	0959-3993
year published	2005
volume	21
issue	8-9
beginning page	1499
ending page	1507
digital object identifier (doi)	10.1007/s11274-005-7370-7
web of science category	Biotechnology & Applied Microbiology
subject category	Biotechnology & Applied Microbiology
unique article identifier	WOS:000234411400028