abstract
Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C-18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (n(c)), higher selectivity () (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (R-s) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs.
keywords
REVERSED-PHASE HPLC; POLYPHENOLS; OLEUROPEIN; 3,4-DIHYDROXYPHENYLGLYCOL; ANTIOXIDANTS; PERFORMANCE; HYDROLYSIS; TYROSOL
subject category
Biochemistry & Molecular Biology; Chemistry
authors
Ferro, MD; Santos, SAO; Silvestre, AJD; Duarte, MF
our authors
Armando Jorge Domingues Silvestre
Full professor
Maria de Fátima Pereira Duarte
Principal Researcher
Sónia Andreia Oliveira Santos
Assistant Researcheracknowledgements
This work was supported by the Program Alentejo 2020, through the European Fund for Regional Development (FEDER) under the scope of OLEAVALOR-Valorizacao das Variedades de Oliveira Portuguesas (ALT20-03-0145-FEDER-000014).