Doping beta-TCP as a Strategy for Enhancing the Regenerative Potential of Composite beta-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats


The present work aims at evaluating the potential gains derived from partially replacing calcium in resorbable beta-tricalcium phosphate (beta-TCP) by two different molar percentages of strontium (5, 10) and zinc (1, 2), concomitantly with a fixed molar percentage (0.5) of manganese. Synthetic granular composite bone filling grafts consisting of doped beta-TCP and an alkali-free bioactive glass were prepared and implanted in similar to 4 mm diameter bone defects drilled in the calvaria of Wistar rats used as animal models. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Non-manipulated bone was used as positive control, while empty defects were used as a negative control group. The von Kossa staining revealed an enhanced new bone formation with increasing doping levels, supporting the therapeutic effects exerted by the doping elements. The percentage of newly formed bone was similar when the defects were filled with autologous bone, BG (previous results) or 3TCP2/7BG, which indicates that the latter two are excellent candidates for replacement of autologous bone as bone regeneration material. This finding confirms that doping with suitable doses of therapeutic ions is a good strategy towards transposing the bone graft materials to biomedical applications in humans.



subject category

Materials Science


Ferreira, MM; Brito, AF; Brazete, D; Pereira, IC; Carrilho, E; Abrantes, AM; Pires, AS; Aguiar, MJ; Carvalho, L; Botelho, MF; Ferreira, JMF

our authors


Thanks are due to CICECO-Aveiro Institute of Materials (UID/CTM/50011/2013) project funded by FEDER funds through the Operational Programme Competitiveness Factors (COMPETE 2020) and the Portuguese Foundation for Science and Technology (FCT). The authors also would like to thank FCT (Portugal) by the financing of PEst-UID/NEU/04539/2013 and FEDER-COMPETE (FCOMP-01-0124-FEDER-028417 and POCI-01-0145-FEDER-007440).

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