Temperature-responsive extraction of violacein using a tuneable anionic surfactant-based system

abstract

A tuneable thermoresponsive system based on combinations of sodium dodecyl sulfate surfactant with tetrabutylammonium chloride salt is presented as an integrated process for the solid-liquid extraction of violacein from bio-engineered Yarrowia lipolytica yeast and its purification by cloud-point separation from contaminant proteins.

keywords

SODIUM DODECYL-SULFATE; DEEP EUTECTIC SOLVENTS; CLOUD POINT; AQUEOUS-SOLUTIONS; TETRAALKYLAMMONIUM BROMIDES; MICELLAR-SOLUTIONS; RECOVERY; COUNTERIONS; BEHAVIOR; REMOVAL

subject category

Chemistry

authors

Schaeffer, N; Kholany, M; Veloso, TLM; Pereira, JL; Ventura, SPM; Nicaud, JM; Coutinho, JAP

our authors

acknowledgements

This work was developed in the scope of the project CICECO - Aveiro Institute of Materials, POCI-01-0145-FEDER-007679 (FCT Ref. UID/CTM/50011/2019). S. P. M. Ventura and M. Kholany acknowledge FCT for the contract IF/00402/2015 and PhD grant SFRH/BD/138413/2018, respectively. Thanks are due to FCT/MCTES for the financial support to CESAM (UID/AMB/50017/2019), through national funds; J. L. Pereira is funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in article 23 of the Decree-Law 57/2016, changed by Law 57/2017.

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