A polynuclear Cu(ii) complex for real time monitoring of mitochondrial cytochromeCrelease during cellular apoptosis


A new amide-imine conjugate, 2-hydroxybenzoic acid-(2-hydroxybenzylidene)-hydrazide (L-1), is employed to prepare a single crystal X-ray structurally characterized poly-nuclear Cu(ii) complex (M1). M1 selectively and spatially interacts with cytochromeC(CytC) to allow fluorescence imaging of intracellular translocation events in living cells. Thus, direct visualization of a CytCtranslocation event during an apoptotic process is achieved for the first time. The binding constant and LOD are 7.52 x 10(4)M(-1)and 34.0 nM, respectively.

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Khanra, S; Ta, S; Paladhi, A; Ghosh, M; Ghosh, S; Hira, SK; Manna, PP; Brandao, P; Felix, V; Das, D

our authors


SK (09/025(0227)/2017-EMR-I) and AP (09/025(0243)/2018EMR-I) acknowledge CSIR (New Delhi) for fellowships. SKH acknowledges DHESTBT, Govt. WB, (No. ST/P/S & T/2G-24/2017) for partial financial support. The SC-XRD facility is supported by the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, and is financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement.

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