abstract
CCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment. In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades.
keywords
CHROMATED COPPER ARSENATE; TREATED WOOD; CHILDRENS EXPOSURE; CELL-CYCLE; CHROMIUM; PRESERVATIVES; COMBINATIONS; DICHROMATE; APOPTOSIS; METALS
subject category
Environmental Sciences & Ecology; Pharmacology & Pharmacy; Toxicology
authors
Matos, RC; Oliveira, H; Fonseca, HMAC; Morais, S; Sharma, B; Santos, C; Pereira, MD
our authors
Helena Oliveira
Post-Doc Fellowship
Maria de Lourdes Gomes Pereira
Associate Professor with Aggregationacknowledgements
This work was held under the scope of the projects CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, CESAM, FCT Ref. UID/AMB/50017/2019, and FCT Ref. UID/GEO/04035/2019, financed by national funds through the FCT/MCTES and when appropriate co -financed by FEDER under the PT2020 Partnership Agreement. Also, FEDER/COMPETE/POCI, (POCI-01-0145-FEDER-006958) and FCT, UID/AGR/04033/2013 are acknowledged. H.O. acknowledges FCT/MCTES for her research contract under the Program Stimulus to Scientific Employment (CEECIND/04050/2017).