|
authors |
Alves, NR; Pereira, MM; Giordano, RLC; Tardioli, PW; Lima, AS; Soares, CMF; Souza, RL |
|
nationality |
International |
|
journal |
BIOPROCESS AND BIOSYSTEMS ENGINEERING |
|
author keywords |
Cross-linked enzyme aggregates; Palm fiber; Burkholderia cepacialipase; Hydrolysis reactions |
|
keywords |
BURKHOLDERIA-CEPACIA; ORGANIC-SOLVENTS; IONIC LIQUIDS; IMMOBILIZATION; LIPASE; POLYETHYLENEIMINE; COAGGREGATION |
|
abstract |
A new design of cross-linked enzyme aggregates (CLEAs) ofBurkholderia cepacialipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 +/- 0.1%) and volumetric activity (1578.1 +/- 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton(R)X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the alpha-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications. |
|
publisher |
SPRINGER |
|
issn |
1615-7591 |
|
isbn |
1615-7605 |
|
year published |
2021 |
|
volume |
44 |
|
issue |
1 |
|
beginning page |
57 |
|
ending page |
66 |
|
digital object identifier (doi) |
10.1007/s00449-020-02419-0 |
|
web of science category |
10 |
|
subject category |
Biotechnology & Applied Microbiology; Engineering, Chemical |
|
unique article identifier |
WOS:000557141100001
|
