Design for preparation of more active cross-linked enzyme aggregates ofBurkholderia cepacialipase using palm fiber residue
authors Alves, NR; Pereira, MM; Giordano, RLC; Tardioli, PW; Lima, AS; Soares, CMF; Souza, RL
nationality International
journal BIOPROCESS AND BIOSYSTEMS ENGINEERING
author keywords Cross-linked enzyme aggregates; Palm fiber; Burkholderia cepacialipase; Hydrolysis reactions
keywords BURKHOLDERIA-CEPACIA; ORGANIC-SOLVENTS; IONIC LIQUIDS; IMMOBILIZATION; LIPASE; POLYETHYLENEIMINE; COAGGREGATION
abstract A new design of cross-linked enzyme aggregates (CLEAs) ofBurkholderia cepacialipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 +/- 0.1%) and volumetric activity (1578.1 +/- 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton(R)X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the alpha-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications.
publisher SPRINGER
issn 1615-7591
isbn 1615-7605
year published 2021
volume 44
issue 1
beginning page 57
ending page 66
digital object identifier (doi) 10.1007/s00449-020-02419-0
web of science category 10
subject category Biotechnology & Applied Microbiology; Engineering, Chemical
unique article identifier WOS:000557141100001
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journal impact factor 2.419
5 year journal impact factor 2.398
category normalized journal impact factor percentile 49.607
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