resumo
A new design of cross-linked enzyme aggregates (CLEAs) ofBurkholderia cepacialipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 +/- 0.1%) and volumetric activity (1578.1 +/- 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton(R)X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the alpha-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications.
palavras-chave
BURKHOLDERIA-CEPACIA; ORGANIC-SOLVENTS; IONIC LIQUIDS; IMMOBILIZATION; LIPASE; POLYETHYLENEIMINE; COAGGREGATION
categoria
Biotechnology & Applied Microbiology; Engineering, Chemical
autores
Alves, NR; Pereira, MM; Giordano, RLC; Tardioli, PW; Lima, AS; Soares, CMF; Souza, RL
nossos autores
Matheus M. Pereira
Investigadoragradecimentos
The authors thank the financial support of FAPITEC/SE, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES)-Finance Code 001; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); and Universidade Tiradentes (PROSUP II-UNIT) for the scholarship to Alves, N.R. The authors also thank Sao Paulo Research Foundation (grant 2016/10636-8) for some kind of financial help.