abstract
Fabrication of biocompatible polymeric carriers for sustained/controlled drug-delivery have been extensively explored over the years. Furthermore, systems based on polymers from natural origins exceed conventional polymers in biocompatibility, biodegradability and cost efficiency. Polysaccharides are one of the most common biopolymers found in nature and they can achieve a high degree of complexity and fine biological properties. Herein, is proposed a biodegradable and biocompatible microcarrier synthesized from laminarin, a low Mw marine polysaccharide based on glucose units with great biological activity, such as immune modulation and antimicrobial properties. Within this work, controlled size microparticles were obtained from novel modifications of laminarin. Microparticles showed 40% release of fluorescein isothiocyanatedextran (70 kDa) after 24 h and full degradability after 11 days, when in physiological conditions. When incubated with human adipose stem and L929 cell lines (up to a microparticle concentration of 100 mu g/mL) no cytotoxicity was perceived, and neither membrane or nucleus disturbance. Thus, microparticles synthesized from laminarin, proved to be a cost efficient, biocompatible and biodegradable system.
keywords
CELL-ADHESION; CANCER; HYDROGEL; MICROSPHERES; FABRICATION; PARTICLES; INDUCTION; APOPTOSIS; ACID
subject category
Chemistry
authors
Castanheira, EJ; Correia, TR; Rodrigues, JMM; Mano, JE
our authors
João M. M. Rodrigues
Assistant Researcher
João Mano
Vice Director
Tiago Ruivo Correia
Junior Researcheracknowledgements
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, and project COP2P (PTDC/QUI-QOR/30771/2017), financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. E.J. Castanheira also acknowledges FCT for his PhD grant (SFRH/BD/144880/2019).