abstract
Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidicsbased separation and purification of proteins and nucleic acids with the focus on liquid-liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.
keywords
AQUEOUS 2-PHASE EXTRACTION; PROTEIN CRYSTALLIZATION CONDITIONS; SELF-INTERACTION CHROMATOGRAPHY; LIQUID-LIQUID-EXTRACTION; SOLID-PHASE EXTRACTION; CAPILLARY-ELECTROPHORESIS; IONIC LIQUIDS; HUMAN SERUM; PROCESS INTENSIFICATION; SERIAL CRYSTALLOGRAPHY
subject category
Chemistry; Science & Technology - Other Topics
authors
Vicente, FA; Plazl, I; Ventura, SPM; Znidarsic-Plazl, P
our authors
Filipa Alexandra André Vicente
PhD Student
Sónia Patrícia Marques Ventura
Assistant Professoracknowledgements
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. S. P. M. Ventura acknowledges FCT for the contract IF/00402/2015 under the Investigador FCT 2015. I. P. and P. Z.-P. were financially supported by the Slovenian Research Agency through Grants P2-0191 and N2-0067, as well as through the European Union's H2020 project COMPETE (Grant 811040), which is gratefully acknowledged.