authors |
Esteves, CIC; Fonseca, ID; Rocha, J; Silva, AMS; Guieu, S |
nationality |
International |
journal |
DYES AND PIGMENTS |
author keywords |
Green fluorescent protein; Aggregation-induced emission enhancement; Synthesis; Fluorescence |
keywords |
GFP-CHROMOPHORE; EMISSION ENHANCEMENT; NITRO |
abstract |
The green fluorescent protein (GFP) is extensively used as a biomarker for fluorescence biological imaging. The chromophore in GFP is only fluorescent when confined into the beta-barrel of the protein. Similarly, synthetic analogues of the fluorophore of GFP are usually non-emissive in solution, due to free rotation around the aryl-alkene bond and (Z/E)-isomerization of the double bond. Here, the synthesis and characterization of three analogues of the fluorophore of GFP are reported. The introduction of more electron donating substituents induces a red-shift in the absorption and emission. The fluomphores are more emissive in the solid state than in solution, and a study of their crystal structure reveals that the (Z/E)-isomerization is efficiently blocked in the crystals. |
publisher |
ELSEVIER SCI LTD |
issn |
0143-7208 |
isbn |
1873-3743 |
year published |
2020 |
volume |
177 |
digital object identifier (doi) |
10.1016/j.dyepig.2020.108267 |
web of science category |
Chemistry, Applied; Engineering, Chemical; Materials Science, Textiles |
subject category |
Chemistry; Engineering; Materials Science |
unique article identifier |
WOS:000525303800018
|