abstract
Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor left-to-right markleft-to-right markbioavailability in the human body, caused by its low aqueous solubility and fast degradation, left-to-right markleft-to-right markpresents a big hurdle for its oral administration. Here, we used nano-vesicles made of left-to-right markleft-to-right markphospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were left-to-right markleft-to-right markencapsulated in the produced phospholipid system to form drug-loaded liposomes. left-to-right markCurcumin's left-to-right markconcentration was evaluated using UV-visible measurements. The maximal left-to-right markamount of curcumin left-to-right markthat could be added to liposomes was assessed. Nuclear magnetic left-to-right markresonance (NMR) analyses left-to-right markwere used to determine curcumin's interactions and localization left-to-right markwithin the phospholipid left-to-right markmembrane of the liposomes. X-ray scattering (SAXS) and atomic left-to-right markforce microscopy (AFM) left-to-right markexperiments were performed to characterize the membrane structure left-to-right markand organization, as well as its left-to-right markmechanical properties at the nanoscale. Conservation of the membrane's properties is found with left-to-right markthe addition of curcumin in various left-to-right markamounts before saturation, allowing the preparation of a left-to-right markdefined nanocarrier with desired left-to-right markamounts of the drug.
keywords
NANOPARTICLES; CURVATURE
subject category
Biochemistry & Molecular Biology; Chemistry
authors
Arab-Tehrany, E; Elkhoury, K; Francius, G; Jierry, L; Mano, JF; Kahn, C; Linder, M
our authors
João Mano
Vice Directoracknowledgements
K.E. acknowledges support from the French Ministry of Higher Education, Research and Innovation. This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES.