In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight
authors Sukul, M; Sahariah, P; Lauzon, HL; Borges, J; Masson, M; Mano, JF; Haugen, HJ; Reseland, JE
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Chitosan; Degree of deacetylation; Molecular weight; Osteoblasts
keywords IONIC-STRENGTH; SCAFFOLD; DIFFERENTIATION; ACETYLATION; ENDOTOXINS; HYDROGELS; DELIVERY; CHITIN; GROWTH; CELLS
abstract We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
publisher ELSEVIER SCI LTD
issn 0144-8617
isbn 1879-1344
year published 2021
volume 254
digital object identifier (doi) 10.1016/j.carbpol.2020.117434
web of science category 11
subject category Chemistry, Applied; Chemistry, Organic; Polymer Science
unique article identifier WOS:000604614900003
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journal analysis (jcr 2019):
journal impact factor 7.182
5 year journal impact factor 6.89
category normalized journal impact factor percentile 96.638
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