In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight

authors	Sukul, M; Sahariah, P; Lauzon, HL; Borges, J; Masson, M; Mano, JF; Haugen, HJ; Reseland, JE
nationality	International
journal	CARBOHYDRATE POLYMERS
author keywords	Chitosan; Degree of deacetylation; Molecular weight; Osteoblasts
keywords	IONIC-STRENGTH; SCAFFOLD; DIFFERENTIATION; ACETYLATION; ENDOTOXINS; HYDROGELS; DELIVERY; CHITIN; GROWTH; CELLS
abstract	We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
publisher	ELSEVIER SCI LTD
issn	0144-8617
isbn	1879-1344
year published	2021
volume	254
digital object identifier (doi)	10.1016/j.carbpol.2020.117434
web of science category	11
subject category	Chemistry, Applied; Chemistry, Organic; Polymer Science
unique article identifier	WOS:000604614900003