Simultaneous separation of egg white proteins using aqueous three-phase partitioning systems
authors Belchior, DCV; Freire, MG
nationality International
journal JOURNAL OF MOLECULAR LIQUIDS
author keywords Egg white proteins; Ovalbumin, Lysozyme; Three phase-partitioning; Aqueous biphasic system; Separation
keywords IONIC LIQUID RECOVERY; 2-PHASE SYSTEMS; PHASE-BEHAVIOR; PURIFICATION; LYSOZYME; OVALBUMIN; EXTRACTION; PROTEASES; OVOTRANSFERRIN; OPTIMIZATION
abstract Egg white proteins (ovalbumin, ovotransferrin, lysozyme, ovoinhibitor and ovomucin) are used in a wide range of applications, thus requiring the development of cost-effective purification platforms for their isolation and recovery. In this work, aqueous biphasic systems (ABS) and three phase partitioning (TPP) were investigated as combined approaches to separate egg white proteins. ABS composed of poly(ethylene)glycol of different molecular weights and potassium phosphate buffer at pH 7 were first characterized and optimized to simultaneously separate ovalbumin and lysozyme. Within the ABS concept, there is the preferential partition of both proteins to the PEG-rich phase. However, by increasing the PEG molecular weight or phase-forming components content, there is the selective precipitation of lysozyme over ovalbumin at the ABS interface, falling within the TPP concept. The best TPP systems were then applied to separate proteins directly from egg white. With the best TPP identified, there is the fractionation of 3 main proteins from egg yolk, namely ovalbumin in the PEG-rich phase, lysozyme at the ABS interface and ovoinhibitor in the salt-rich phase. The obtained results reinforce the potential of ABS/TTP combined strategies to fractionate high-value proteins from complex matrices in a single-step. (C) 2021 Elsevier B.V. All rights reserved.
publisher ELSEVIER
issn 0167-7322
isbn 1873-3166
year published 2021
volume 336
digital object identifier (doi) 10.1016/j.molliq.2021.116245
web of science category 9
subject category Chemistry, Physical; Physics, Atomic, Molecular & Chemical
unique article identifier WOS:000664654300032
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 5.065
5 year journal impact factor 4.766
category normalized journal impact factor percentile 81.277
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg