abstract
Guava seed biochar appears as a new alternative of the effective support to the immobilization ofBurkholderia cepacialipase (BCL) by physical adsorption. The objective of this work was to evaluate the potential of this immobilized biocatalyst in the transesterification reaction of crude coconut oil and ethanol and to understand the mechanism of the reaction through the study of molecular docking. The best loading of BCL was determined to be 0.15 g(enzyme)/g(support)having a hydrolytic activity of 260 U/g and 54% immobilization yield. The products of transesterification reaction produced a maximum yield at 40 degrees C under different reaction conditions. The monoacylglycerols (MAGs) conversion of 59% was using substrate molar ratio oil:ethanol of 1:7 with the reaction time of 24 H. In addition, the highest ethyl esters yield (48%) had the molar ratio of 1:7 with the reaction time of 96 H and maximum conversion of diacylglycerols (DAGs) was 30% with the molar ratio of 1:6 with the reaction time of 24 H. Molecular Docking was applied to clarify the mechanisms of transesterification reaction at the molecular level. MAGs and DAGs are compounds with excellent emulsifying properties used in industrial production of several bioproducts such as cosmetic, pharmaceuticals, foods, and lubricants.
keywords
CANDIDA-RUGOSA LIPASE; BIODIESEL PRODUCTION; ADSORPTION; GLYCEROLYSIS; SUPPORTS; LIQUID; SILICA; CORE; SOIL
subject category
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
authors
Almeida, LC; Barbosa, MS; de Jesus, FA; Santos, RM; Fricks, AT; Freitas, LS; Pereira, MM; Lima, AS; Soares, CMF
our authors
Matheus M. Pereira
Researcheracknowledgements
The authors acknowledge financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) (88887357049/2019-00), Fundacao de Apoio a Pesquisa e Inovacao Tecnologica do Estado de Sergipe (FAPITEC), and Universidade Tiradentes (UNIT).