abstract
The separation and purification of biologically-active compounds from natural sources is of interest because such molecules find wide application in the pharmaceutical sector and in other industrial areas. Roselle (Hibiscus sabdariffa) plants are a good source of anthocyanins, flavonoids, hydroxycitric acid, tartaric acid, ascorbic acid and hibiscus acid. The separation of hibiscus acid from the Roselle extract is very challenging, requiring the use of selective methods. It is accomplished here by means of the indium-bearing Metal Organic Framework MIL-68-NH2. Before and after exposure to MIL-68NH(2), the Roselle extract is analyzed by thin-layer chromatography, ultraviolet-visible spectrophotometry, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The structural integrity of MIL-68-NH2 after the separations is investigated by powder X-ray diffraction, nuclear magnetic resonance and infrared spectroscopy, confirming the adsorption selectivity of MIL-68-NH2 towards hibiscus acid. (C) 2020 Elsevier B.V. All rights reserved.
keywords
HYDROXYCITRIC ACID; SABDARIFFA L.; FRUIT PECTINS; LACTONE; POLYHYDROXYPHENOLS; ANTHOCYANIN
subject category
Biochemical Research Methods; Chemistry, Analytical
authors
Abdelhameed, RM; Rocha, J; Silva, AMS
our authors
João Rocha
Full Professor
Reda Mohamed Abdelhameed Ahmed
Post-doc Fellowshipacknowledgements
Thanks are due to FCT/MEC for the financial support the QOPNA research Unit (FCT UID/QUI/0 0062/2019) and CICECO -Aveiro Institute of Materials (UIDB/50011/2020 & UIDP/50011/2020) through national founds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network.