Recovery of lactose and proteins from cheese whey with poly(ethylene) glycol/sulfate aqueous two-phase systems
authors Gonzalez-Amado, M; Tavares, APM; Freire, MG; Soto, A; Rodriguez, O
nationality International
journal SEPARATION AND PURIFICATION TECHNOLOGY
author keywords Aqueous two-phase systems; Cheese whey; Bovine serum albumin; beta-lactoglobulin; alpha-lactalbumin; Separation
abstract Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, beta-lactoglobulin and alpha-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200-8000 g.mol(-1)) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes.
publisher ELSEVIER
issn 1383-5866
isbn 1873-3794
year published 2021
volume 255
digital object identifier (doi) 10.1016/j.seppur.2020.117686
web of science category 10
subject category Engineering, Chemical
unique article identifier WOS:000633046200003
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journal impact factor 5.774
5 year journal impact factor 5.257
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