Comparison of the Photodynamic Action of Porphyrin, Chlorin, and Isobacteriochlorin Derivatives toward a Melanotic Cell Line
authors Castro, KADF; Ramos, L; Mesquita, M; Biazzotto, JC; Moura, NMM; Mendes, RF; Paz, FAA; Tome, AC; Cavaleiro, JAS; Simoes, MMQ; Faustino, MAF; Jager, AV; Nakagaki, S; Neves, MGPMS; Da Silva, RS
nationality International
journal ACS APPLIED BIO MATERIALS
author keywords porphyrin; chlorin; isobacteriochlorin; melanoma; PDT
keywords SUBCELLULAR-LOCALIZATION; PHOTOPHYSICAL PROPERTIES; THERAPY; CANCER; PEROXYNITRITE; MECHANISMS; PHOTOBIOMODULATION; BACTERIOCHLORINS; PHOTOSENSITIZERS; TYROSINE
abstract Melanoma is the most dangerous form of skin cancer, with an abrupt growth of its incidence over the last years. It is extremely resistant to traditional treatments such as chemotherapy and radiotherapy, but therapies for this cancer are gaining attention. Photodynamic therapy (PDT) is considered an effective modality to treat several types of skin cancers and can offer the possibility to treat one of the most aggressive ones: melanoma. In this work, the effect of PDT on a melanotic cell line (B16F10 cells) was assessed by exposing cultured cells to 5,10,15-tris(pentafluorophenyl)-20-(4-pyridyl)porphyrin (PS1) and to its chlorin (PS2) and isobacteriochlorin (PS3) corresponding derivatives and red LED light (lambda = 660 +/- 20 nm). The PDT effect in the cells' viability was measured using the MTT assay. The cell apoptosis was quantified by flow cytometry, and the subcellular localization of the photosensitizer was determined by fluorescence microscopy. In addition, the ability of PS2 to generate superoxide radicals was qualitatively assessed by tyrosine nitration. The results show that the efficiency of the PDT process is dependent on the structure of the PS and on their ability to produce singlet oxygen. Besides that, the photoactivation efficiency is highly dependent on the cellular sublocalization of the PS and on its cellular uptake and singlet oxygen production. We also found that the resistant cell line B16F10 has distinctive chlorin, isobacteriochlorin, or porphyrin-specific resistance profiles. Furthermore, it is shown that the highly fluorescent chlorin derivative PS2 can also be considered in imaging diagnostics.
publisher AMER CHEMICAL SOC
issn 2576-6422
year published 2021
volume 4
issue 6
beginning page 4925
ending page 4935
digital object identifier (doi) 10.1021/acsabm.1c00218
web of science category 11
subject category Nanoscience & Nanotechnology; Materials Science, Biomaterials
unique article identifier WOS:000664594300024

Sponsors

1suponsers_list_ciceco.jpg