Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
authors de Sousa, BM; Correia, CR; Ferreira, JAF; Mano, JF; Furlani, EP; dos Santos, MPS; Vieira, SI
nationality International
journal NPJ REGENERATIVE MEDICINE
keywords TOTAL HIP-ARTHROPLASTY; ELECTRICAL-STIMULATION; EXTRACELLULAR VESICLES; ORTHOPEDIC IMPLANTS; KNEE REPLACEMENT; MATRIX VESICLES; GENE-EXPRESSION; BONE-FORMATION; UP-REGULATION; STEM-CELLS
abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial osseointegration but lack long-term personalized actuation on the bone-implant interface. Novel bioelectronic devices comprising biophysical stimulators and sensing systems are thus emerging, aiming for long-term control of peri-implant bone growth through biointerface monitoring. These acting-sensing dual systems require high frequency (HF) operations able to stimulate osteoinduction/osteoconduction, including matrix maturation and mineralization. A sensing-compatible capacitive stimulator of thin interdigitated electrodes and delivering an electrical 60 kHz HF stimulation, 30 min/day, is here shown to promote osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). HF stimulation through this capacitive interdigitated system had significant effects on osteoblasts' collagen-I synthesis, matrix, and mineral deposition. A proteomic analysis of microvesicles released from electrically-stimulated osteoblasts revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). Proteomics data are available via ProteomeXchange with the identifier PXD028551. Further, under HF stimulation, hASCs exhibited higher osteogenic commitment and enhanced hydroxyapatite deposition. These promising osteoinductive/conductive capacitive stimulators will integrate novel bioelectronic implants able to monitor the bone-implant interface and deliver personalized stimulation to peri-implant tissues.
publisher NATURE PORTFOLIO
isbn 2057-3995
year published 2021
volume 6
issue 1
digital object identifier (doi) 10.1038/s41536-021-00184-6
web of science category 14
subject category Cell & Tissue Engineering; Engineering, Biomedical
unique article identifier WOS:000721880800001
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journal analysis (jcr 2019):
journal impact factor 7.021
5 year journal impact factor 8.298
category normalized journal impact factor percentile 90.805
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