Recovery of bacterioruberin and proteins using aqueous solutions of surface-active compounds


Haloarchaea microorganisms are little explored marine resources that can be a promising source of valuable compounds with unique characteristics, due to their adaptation to extreme environments. In this work, the extraction of bacterioruberin and proteins from Haloferax mediterranei ATCC 33500 was investigated using aqueous solutions of ionic liquids and surfactants, which were further compared with ethanol. Despite the good performance of ethanol in the extraction of bacterioruberin, the use of aqueous solutions of surface-active compounds allowed the simultaneous release of bacterioruberin and proteins in a multi-product process, with the non-ionic surfactants being identified as the most promising. The optimum operational conditions allowed a maximum extraction yield of 0.37 +/- 0.01 mg(bacterioruberin) g(wet biomass)(-1) and 352 +/- 9 mg(protein) g(wet biomass)(-1) with an aqueous solution of Tween (R) 20 (at 182.4 mM) as the extraction solvent. In addition, high purities of bacterioruberin were obtained, after performing a simple induced precipitation using ethanol as an antisolvent to recover the proteins present in the initial extract. Finally, a step for polishing the bacterioruberin was performed, to enable solvent recycling, further closing the process to maximize its circularity.



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Vaz, BMC; Kholany, M; Pinto, DCGA; Macario, IPE; Veloso, T; Caetano, T; Pereira, JL; Coutinho, JAP; Ventura, SPM

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This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020,.nanced by national funds through the FCT/MEC (PIDDAC). The authors acknowledge FTC/MCTES for the.nancial support to CESAM, UIDB/50017/2020 + UIDP/50017/2020 + LA/P/0094/2020,.nanced by national funds. The authors are also grateful to the FCT for the doctoral grants of M. Kholany (SFRH/BD/138413/2018), I. P. E. Mac ' ario (SFRH/BD/123850/2016), and T. Veloso (SFRH/BD/147346/2019). T. Caetano was funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July (CEECIND/01463/2017). The National NMR Network, funded within the framework of the National Program for Scienti.c Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT, is also acknowledged.

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