abstract
Currently, the ability to produce several kinds of water-soluble red natural colorants makes the genus Talaromyces particularly important to the dye industry, which can be an alternative to the use of harmful synthetic colorants. In this study, colored compounds produced by Talaromyces amestolkiae were extracted, characterized chemically and the color stability of the fermented broth without any extraction procedure was further evaluated over pH variation. Five azaphilones compounds were detected by Ultrahigh Performance Liquid Chromatography-Mass Spectrometry system, all being complexes of the fatty acid amino-hexanedioic acid and azaphilone Monascus colorants. The color of the fermented broth was stable at a wide range of pH (3-9). Furthermore, T. amestolkiae colorants precipitated through hydrolysis of key chemical groups at extremely acidic (pH 1) and lose red color in extremely basic (pH 13) medium, showing negative halochromism. Nevertheless, these findings enhance the industrial relevance of azaphilone colorants produced by biotechnological process.
keywords
SUBMERGED CULTURE-CONDITIONS; CITRININ PRODUCTION; FUNGAL PIGMENTS; RED; BIOSYNTHESIS; CHEMISTRY; QUALITY; ORANGE; PH
subject category
Chemistry; Food Science & Technology; Nutrition & Dietetics
authors
de Oliveira, F; Rocha, ILD; Pinto, DCGA; Ventura, SPM; dos Santos, AG; Crevelin, EJ; Ebinuma, VDS
our authors
Sónia Patrícia Marques Ventura
Assistant Professoracknowledgements
This work was developed within the scope of the project CICECOAveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. Thanks are due to the University of Aveiro and FCT/MCT for the financial support of LAQV-REQUIMTE (UIDB/50006/2020); Val ' eria C. Santos-Ebinuma and Fernanda de Oliveira acknowledge FAPESP (2014/01580-3, 2018/06908-8, 2019/15493-9), CNPq (National Council for Scientific and Technological Development, Brazil) and CAPES (Coordination of Superior Level Staff Improvement, Brazil) -Finance Code 001.