abstract
Marine microalgae are promising sources of novel valuable biomolecules such as polysaccharides. In this study, the dinoflagellate Heterocapsa sp. AC210 was described as a new exopolysaccharide (EPS) producer. The culti-vation and EPS production in bioreactor was evaluated for the first time in detail. The EPS was composed of seven different sugar monomers, including fucose and glucosamine, which are quite rare and have never been reported in dinoflagellates' EPS. Moreover, the EPS had a high content of sulphate, which is often associated with biological properties. Cytotoxicity was accessed and the results showed that the EPS did not reduce cell viability for concentrations up to 1 g L-1. Additionally, antioxidant and anti-inflammatory assays demonstrated that the EPS reduced by 18 % the intracellular reactive oxygen species and decreased up to 79.3 % and 46.2 % of IL-8 and IL-6 secretion in keratinocytes, which supports its potential application in the cosmeceutical and biomedical fields.
keywords
EXTRACELLULAR POLYMERIC SUBSTANCES; SULFATED EXOPOLYSACCHARIDE; PECTIC POLYSACCHARIDE; MICROALGAL; EXTRACTION; ALGAE
subject category
Biotechnology & Applied Microbiology
authors
Concórdio-Reis, P; Cardeira, M; Macedo, AC; Ferreira, SS; Serra, AT; Coimbra, MA; Amorim, A; Reis, MAM; Freitas, F
our authors
Sónia dos Santos Ferreira
Post-Doc FellowshipProjects
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was supported by Research Unit on Applied Molecular Biosciences - UCIBIO (UIDP/04378/2020 and UIDB/04378/2020) , Associate Laboratory Institute for Health and Bioeconomy-i4HB (LA/P/0140/202019) , CICECO (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) , LAQV/REQUIMTE (UIDB/50006/2020, UIDP/50006/2020) , and iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) , through national funds from FCT-Funda?a?o para a Cie?ncia e a Tecnologia, I.P. and, where applicable, co-financed by the FEDER-Fundo Europeu de Desenvolvimento Regional, within the PT2020 Partnership Agreement. The Associate Laboratories LS4FUTURE (LA/P/0087/2020) are also acknowledged. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC) , is gratefully acknowledged. This work was also supported by FCT, through the strategic project UIDB/04292/2020 awarded to MARE and through project LA/P/0069/2020 granted to the Associate Laboratory ARNET. P. C.-R. acknowledges FCT I.P. for PhD Grant SFRH/BD/131947/2017. ATS also acknowledges FCT/Ministerio da CieIncia, Tecnologia e Ensino Superior for the Individual Grant CEEC-IND/04801/2017.