Functional changes of mice Sertoli cells induced by Cr(V)
authors Pereira, MD; Santos, TM; Costa, FGE; de Jesus, JP
nationality International
journal CELL BIOLOGY AND TOXICOLOGY
author keywords blood-testis barrier; Cr(V); horseradish peroxidase; Sertoli cell; testis damage
keywords MODEL CHROMIUM(V) COMPLEX; TOXICITY; MECHANISMS; APOPTOSIS; HAZARDS; DAMAGE; DNA
abstract Transport of macromolecules from the interstitial testis tissue to cells at the adlumenal compartment of the seminiferous epithelium occurs naturally through Sertoli cells. In previous studies we have shown that Cr(V) intoxication disturbed spermatogenesis in mice. To test if Sertoli cells are affected by chromium, a well proved carcinogen, the uptake and the horseradish peroxidase transport ability of isolated seminiferous tubules of mice administered with a chromium(V) compound, have been studied. Male CD-R mice were exposed daily for 5 days to [Cr-V-BT](2-) through subcutaneous injection and comparisons were made with groups of vehicle-treated mice. Using an in vitro assay we demonstrated that the seminiferous tubules were able to uptake and transport the tracer, in a much faster way than controls, mainly via intercellular and transcellular pathways, providing evidence that this functional role of Sertoli cells is affected by the Cr(V) compound. These findings might improve the knowledge on the toxicity mechanisms of chromium.
publisher SPRINGER
issn 0742-2091
year published 2004
volume 20
issue 5
beginning page 285
ending page 291
web of science category Cell Biology; Toxicology
subject category Cell Biology; Toxicology
unique article identifier WOS:000226368900003
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 6.284
5 year journal impact factor 4.276
category normalized journal impact factor percentile 87.713
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg