Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

abstract

The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C(8)mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.

keywords

PHASE-DIAGRAM DATA; BIPHASIC SYSTEMS; EXTRACTIVE FERMENTATION; PARTITIONING BEHAVIOR; POLYETHYLENE-GLYCOL; POLYACRYLAMIDE-GELS; MOLECULAR-WEIGHT; AMINO-ACIDS; PROTEINS; IMIDAZOLIUM

subject category

Chemistry; Science & Technology - Other Topics

authors

Ventura, SPM; de Barros, RLF; Barbosa, JMD; Soares, CMF; Lima, AS; Coutinho, JAP

our authors

acknowledgements

The authors are grateful for financial support from Fundacao para a Ciencia e a Tecnologia for the Ph.D. grant SFRH/BD/37830/2007 of S.P.M. Ventura. Moreover, the authors are grateful for the financial support from CAPES for the Ph.D. grant 551223/2010-3 of Jose M. P. Barbosa and grant PIBIC from FAPITEC for the grant of Rafael L. F. de Barros.

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