resumo
Hydroxyapatite (Ca-10(PO4)(6)(OH)(2) - HAp) is a common material for bone replacement in artificial implants and prostheses, due to its high biocompatibility. In this work we report about the preparation of HAp-based materials of marine origin with antibacterial properties. Bones from cod fish (Gadhus morua) were treated with a dilute AgNO3 solution, to obtain HAp-containing samples with silver as a minor component. The samples were characterised by XRD, FT-IR spectroscopy and SEM, indicating the presence of HAp, beta-TCP and some metallic silver. The determination of unit cell parameters, however, indicated that the majority of silver is substituted in the beta-TCP lattice in ionic form. The antibacterial activity of these materials was tested towards both Gram-positive and Gram-negative bacterias; results showed that the material is very effective with Gram-negative strains such as Escherichia coli (inactivation rates of 99.82% and 99.999% after 2 and 5 h, respectively) and had a smaller but still significant effect on Gram-positive MRSA (91% inactivation rate). These results show the potentials of these samples as infection-resistant bone replacement materials. (C) 2015 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
palavras-chave
BETA-TRICALCIUM PHOSPHATE; SUBSTITUTED HYDROXYAPATITE; BIOFILM FORMATION; FISH BONES; BIOCOMPATIBILITY; COMPOSITES; COATINGS; RELEASE; APATITE; NANOHYDROXYAPATITE
categoria
Materials Science
autores
Piccirillo, C; Pullar, RC; Tobaldi, DM; Castro, PML; Pintado, MME
nossos autores
David Maria Tobaldi
Investigador
Rob Pullar
Colaboradoragradecimentos
This work was supported through the PEst-OE/EQB/LA0016/2013, developed within the iCOD project (Inovadora Tecnologias para a Valorizacao de Subprodutos do Processamento do Bacalhau, contract QREN AdI 11466) and in the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID /CTM /50011/2013), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement. C. Piccirillo and R.C. Pullar thank FCT (Portugal) for their research Grant (SFRH/BPD/86483/2012 and SFRH/BPD/97115/2013, respectively).