resumo
The increasing interest in the effect of strontium in bone tissue repair has promoted the development of bioactive materials with strontium release capability. According to literature, hybrid materials based on the system PDMS-SiO2 have been considered a plausible alternative as they present a mechanical behavior similar to the one of the human bone. The main purpose of this study was to obtain a biocompatible hybrid material with simultaneous calcium and strontium release capability. A hybrid material, in the system PDMS-SiO2-CaO-SrO, was prepared with the incorporation of 0.05 mol of titanium per mol of SiO2. Calcium and strontium were added using the respective acetates as sources, following a sol-gel technique previously developed by the present authors. The obtained samples were characterized by FT-IR, solid-state NMR, and SAXS, and surface roughness was analyzed by 3D optical profilometry. In vitro studies were performed by immersion of the samples in Kokubo's SBF for different periods of time, in order to determine the bioactive potential of these hybrids. Surfaces of the immersed samples were observed by SEM, EDS and PIXE, showing the formation of calcium phosphate precipitates. Supernatants were analyzed by ICP, revealing the capability of the material to simultaneously fix phosphorus ions and to release calcium and strontium, in a concentration range within the values reported as suitable for the induction of the bone tissue repair. The material demonstrated to be cytocompatible when tested with MG63 osteoblastic cells, exhibiting an inductive effect on cell proliferation and alkaline phosphatase activity. (C) 2016 Elsevier B.V. All rights reserved.
palavras-chave
MESENCHYMAL STROMAL CELLS; SMALL-ANGLE SCATTERING; SOL-GEL MATERIALS; BIOACTIVE GLASSES; IN-VITRO; OSTEOGENIC DIFFERENTIATION; GAMMA-IRRADIATION; STEM-CELLS; PROLIFERATION; RANELATE
categoria
Materials Science
autores
Almeida, JC; Wacha, A; Gomes, PS; Alves, LC; Fernandes, MHV; Salvado, IMM; Fernandes, MHR
nossos autores
Grupos
G3 - Materiais Eletroquímicos, Interfaces e Revestimentos
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
This work was financed by the JECS Trust (contract 201467), FEDER funds (Program COMPETE) and by FCT funds, by the grant SFRH/BD/72074/2010 and also developed in the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM /50011/2013), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement.