resumo
Developing complex supramolecular biomaterials through highly dynamic and reversible noncovalent interactions has attracted great attention from the scientific community aiming key biomedical and biotechnological applications, including tissue engineering, regenerative medicine, or drug delivery. In this study, the authors report the fabrication of hybrid supramolecular multilayered biomaterials, comprising high-molecular-weight biopolymers and oppositely charged low-molecular-weight peptide amphiphiles (PAs), through combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly approach. Alginate, an anionic polysaccharide, is used to trigger the self-assembling capability of positively charged PA and formation of 1D nanofiber networks. The LbL technology is further used to fabricate supramolecular multilayered biomaterials by repeating the alternate deposition of both molecules. The fabrication process is monitored by quartz crystal microbalance, revealing that both materials can be successfully combined to conceive stable supramolecular systems. The morphological properties of the systems are studied by advanced microscopy techniques, revealing the nanostructured dimensions and 1D nanofibrous network of the assembly formed by the two molecules. Enhanced C2C12 cell adhesion, proliferation, and differentiation are observed on nanostructures having PA as outermost layer. Such supramolecular biomaterials demonstrate to be innovative matrices for cell culture and hold great potential to be used in the near future as promising biomimetic supramolecular nanoplatforms for practical applications.
palavras-chave
QUARTZ-CRYSTAL MICROBALANCE; POLYELECTROLYTE MULTILAYERS; BIOMEDICAL APPLICATIONS; SECONDARY STRUCTURE; POLYMER-FILMS; OSTEOGENIC DIFFERENTIATION; INFRARED-SPECTROSCOPY; REGENERATIVE MEDICINE; CIRCULAR-DICHROISM; SURFACE INTERFACE
categoria
Chemistry; Science & Technology - Other Topics; Materials Science; Physics
autores
Borges, J; Sousa, MP; Cinar, G; Caridade, SG; Guler, MO; Mano, JF
nossos autores
João Borges
Investigador Auxiliar
João Mano
Vice-Diretor
Maria Sousa
Estudante de doutoramento
Sofia Caridade
Bolseiro de pós-Doutoramentoagradecimentos
The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the Postdoctoral (SFRH/BPD/103604/2014-Joao Borges; SFRH/BPD/96797/2013-Sofia G. Caridade) and PhD (SFRH/BD/97606/2013-Maria P. Sousa) grants. This work was also supported by the European Research Council Grant Agreement ERC-2014-ADG-669858 for project "ATLAS", as well as by the H2020-TWINN-2015 - Twinning European project "CHEM2NATURE" (Grant Agreement no. 692333). The Acknowledgements were modified to include cover funding on May 4, 2017, following initial online publication.