Control of Listeria innocua biofilms by biocompatible photodynamic antifouling chitosan based materials

authors	Castro, KADF; Moura, NMM; Fernandes, A; Faustino, MAF; Simoes, MMQ; Cavaleiro, JAS; Nakagaki, S; Almeida, A; Cunha, A; Silvestre, AJD; Freire, CSR; Pinto, RJB; Neves, MDPMS
nationality	International
journal	DYES AND PIGMENTS
author keywords	Porphyrins; Chitosan films; Photodynamic inactivation; Listeria innocua; Biofilms
keywords	IN-VITRO; ANTIBACTERIAL ACTIVITY; PACKAGING MATERIAL; INACTIVATION; PORPHYRINS; THERAPY; PHOTOINACTIVATION; PHOTOSENSITIZERS; DERIVATIVES; FILMS
abstract	New materials obtained through the incorporation of meso-tetraarylporphyrins, bearing phenyl or pentafluorophenyl groups at the meso positions with or without acid groups, in chitosan films have been prepared and characterized in detail. The efficiency of these porphyrinic-chitosan films (PS-CF) to prevent Listeria innocua attachment and subsequent biofilm formation was evaluated under different irradiation protocols (dark, light, light + dark, dark + light). All tested porphyrinic-chitosan films were photostable and effective in reducing Listeria innocua attachment during a period of 24 h exposure to white light. Biofilm development was nearly completely inhibited in two of the PS-CF films (one containing the 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin and the other one of the thio-carboxylate porphyrins) after being exposed to white light for 24 h followed by incubation in the dark for 48 h. Experiments with the non-immobilized porphyrins against cell suspensions of the same Listeria innocua strain showed that, the photodynamic inactivation efficiency was dependent on porphyrin. The demonstrated antimicrobial activity of PS-CF films, associated with the easy recovery of these films, make them promising photodynamic antifouling materials. (C) 2016 Elsevier Ltd. All rights reserved.
publisher	ELSEVIER SCI LTD
issn	0143-7208
isbn	1873-3743
year published	2017
volume	137
beginning page	265
ending page	276
digital object identifier (doi)	10.1016/j.dyepig.2016.10.020
web of science category	Chemistry, Applied; Engineering, Chemical; Materials Science, Textiles
subject category	Chemistry; Engineering; Materials Science
unique article identifier	WOS:000390182700031