High throughput characterization of structural differences between closely related proteins in solution
authors Zaslavsky, A; Madeira, P; Breydo, L; Uversky, VN; Chait, A; Zaslavsky, B
nationality International
journal BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
author keywords Partitioning; Protein structure; Aqueous two-phase system; Structural change; Solvent interaction analysis
keywords AQUEOUS 2-PHASE SYSTEMS; SOLVATION ENERGY RELATIONSHIP; VOLATILE ORGANIC-COMPOUNDS; SOLVENT PROPERTIES; BIPHASIC SYSTEMS; POLYETHYLENE-GLYCOL; METHYLENE GROUP; DRUGS; PARTITION; PEPTIDES
abstract Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired. (C) 2012 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 1570-9639
year published 2013
volume 1834
issue 2
beginning page 583
ending page 592
digital object identifier (doi) 10.1016/j.bbapap.2012.11.004
web of science category Biochemistry & Molecular Biology; Biophysics
subject category Biochemistry & Molecular Biology; Biophysics
unique article identifier WOS:000314616900011
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journal analysis (jcr 2019):
journal impact factor 2.371
5 year journal impact factor 2.539
category normalized journal impact factor percentile 40.542
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