resumo
Bioseparation processes are a relevant part of modern biotechnology, particularly regarding the development of efficient and biocompatible methods for the separation and purification of added-value biologically active compounds. In this field, ionic liquids (ILs) have been proposed, either in liquid-liquid extractions, in which non-water miscible ILs or aqueous biphasic systems (ABS) formed by ILs can be used, or in solid-liquid extractions, in which they are covalently attached to create supported IL phases (SILPs). Aprotic ILs possess unique properties, such as non-volatility and designability, which are valuable in their use in bioseparation processes. In this chapter, we summarize and discuss bioseparation processes based on ILs, including both liquid-liquid and solid-liquid extractions, applied to amino acids and proteins. The most recent and remarkable advances in this area are emphasized, and improvements brought by the use of ILs properly discussed. New insights and envisaged directions with IL-based bioseparation processes are suggested. [GRAPHICS] .
palavras-chave
AQUEOUS 2-PHASE SYSTEMS; SOLID-PHASE EXTRACTION; BOVINE SERUM-ALBUMIN; BIPHASIC SYSTEMS; AMINO-ACIDS; CYTOCHROME-C; PARTITIONING BEHAVIOR; 1-BUTYL-3-METHYLIMIDAZOLIUM BROMIDE; EFFECTIVE SEPARATION; ENHANCED EXTRACTION
categoria
Biotechnology & Applied Microbiology; Engineering
autores
Belchior, DCV; Duarte, IF; Freire, MG
nossos autores
Iola Melissa Fernandes Duarte
Investigador Principal
Mara Guadalupe Freire Martins
Investigador CoordenadorProjectos
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
Igy Technology: A Purication Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IGYPURTECH)
agradecimentos
This work was developed in the scope of projects CICECO - Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013) and MultiBiorefinery (POCI-01-0145-FEDER-016403), financed by national funds through Fundacao para a Ciencia e a Tecnologia (FCT, Portugal)/MEC and co-financed by FEDER under the PT2020 Partnership agreement. The authors acknowledge financial support from the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No 739572 - The Discoveries CTR. D.C.V. Belchior acknowledges financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq for the PhD grant [202337/2015-4]). I.F. Duarte acknowledges the FCT/MCTES for a research contract under the Program 'Investigador FCT'. M.G. Freire acknowledges the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no 337753.