Mesoporous Metal-Organic Frameworks as Effective Nucleating Agents in Protein Crystallography


Mesoporous metal-organic frameworks (mesoMOFs) have gained some attention as protein immobilization agents. Herein, and for the first time, three model proteins (lysozyme, trypsin, and albumin) were used to show that crystallization is effectively induced when the protein is immobilized inside the mesoMOF, with this occurring for lysozyme, while trypsin and albumin were size-excluded. It is shown that heterogeneous nucleation is truly remarkable in the case of lysozyme with a strong adhesion to the mesoMOF surface. These findings suggest that the ability to fix the target protein with a molecular-scale periodicity constitutes a significant advantage of mesoMOFs over other nucleating agents.




Chemistry; Crystallography; Materials Science


Leite, JP; Rodrigues, D; Ferreira, S; Figueira, F; Paz, FAA; Gales, L

nossos autores


This work was financed by Norte-01-0145-FEDER-000008-Porto Neurosciences and Neurologic Disease Research Initiative at I3S, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, by COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, through the European Regional Development Fund (FEDER), and by Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Ensino Superior in the framework of the project Institute for Research and Innovation in Health Sciences (POCI-01-0145-FEDER-007274) and Grant SFRH/BD/129921/2017. This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials, POCI-01-0145-FEDER-007679 (FCT ref. UID/CTM/50011/2013), financed by national funds through the FCT/MEC and when appropriate cofinanced by FEDER under the PT2020 Partnership Agreement.

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