resumo
Ionic liquids (ILs) as adjuvants in polymer-salt aqueous two-phase systems (ATPS) have been used to improve the extraction of biomolecules. However, the impact of Its as adjuvants on the partition of biomolecules is still poorly understood. Previous works mostly focused on ATPS based on strong salting-out agents, which may mask the IL effect. In this work, ATPS formed by polyethylene glycol (PEG 400) and a weak salting-out salt ((NH4)(2)SO4) with a wide number of Its as adjuvants (chloride-based combined with cholinium, imidazolium, pyrrolidinium, piperidinium, tetralkylammonium and tetralkylphosphonium cations) were investigated. The respective phase diagrams were determined, and the systems extraction performance for a wide range of biomolecules (phenolic compounds, alkaloids and amino acids) was investigated. The results obtained show that ILs as adjuvants in polymer-salt ATPS modulate the partition of biomolecules. In particular, more hydrophobic ILs significantly enhance the partition of more hydrophobic biomolecules to the PEG-rich phase (where the IL is enriched). Furthermore, the intensity of the IL effect is more pronounced when using weak salting-out agents. A linear correlation between the biomolecules and the Its partition coefficients, and with the biomolecules octanol-water partition coefficients, was found. In most ATPS formed by polymers and salts using ILs as adjuvants, the biomolecules partition is driven by the Its partition and by the difference in hydrophobicity between the coexisting phases.
palavras-chave
BIPHASIC SYSTEMS; EXTRACTION CAPABILITY; CONTROLLED PH; PURIFICATION; SEPARATION; BOOST
categoria
Biotechnology & Applied Microbiology; Engineering
autores
Neves, CMSS; Sousa, RDS; Pereira, MM; Freire, MG; Coutinho, JAP
nossos autores
Catarina Maia Seco Seiça Neves
Investigador Auxiliar
Mara Guadalupe Freire Martins
Investigador Coordenador
Matheus M. Pereira
Investigador
Rita de Cássia Superbi de Sousa
Bolseiro de pós-DoutoramentoGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
G6 - Materiais Virtuais e Inteligência Artificial
agradecimentos
This work was developed in the scope of the project CICECO-Aveiro Institute of Materials POCI-01-0145- FEDER-007679 (Ref. FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and co-financed by FEDER under the PT2020 Partnership Agreement, and the Multibiorefinery (POCI-01-0145-FEDER-016403) project. C. M. S. S. Neves acknowledges FCT for the postdoctoral grant SFRH/BPD/109057/2015. M. M. Pereira acknowledges the PhD grant (2740-13-3) and financial support from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Capes. Rita de Cassia S. Sousa acknowledges the Post-doctoral grant (200833/2015-4/PDE) and financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq. M. G. Freire acknowledges the European Research Council (ERC) for the Starting Grant ECR-2013-StG-337753.