An Overview on the Recent Advances in Alternative Solvents as Stabilizers of Proteins and Enzymes

resumo

Currently, the use of alternative solvents is increasing, namely ionic liquids (ILs) and deep eutectic solvents (DESs) in diverse fields of knowledge, such as biochemistry, chemistry, chemical engineering, biotechnology and biomedicine. Particularly, when compared to traditional solvents, these alternative solvents have great importance for biomolecules due to the enhanced solubility, structure stability and the biological activity of biomolecules, such as protein and enzymes. Thus, in this review article, the recent developments and efforts on the technological developments carried out with ILs and DESs for the stabilization and activation of proteins and enzymes are provided. The most studied IL- and DES-based formulations for proteins and enzymes are discussed and the molecular mechanisms and interactions related to the increased stability promoted by these alternative solvents are disclosed, while emphasizing their main advantages.

palavras-chave

DEEP-EUTECTIC-SOLVENTS; AQUEOUS BIPHASIC SYSTEMS; IONIC LIQUIDS; THERMAL-STABILITY; SERUM-ALBUMIN; CHALLENGES; BINDING; ANTIBODIES; INSIGHT; LACCASE

categoria

Engineering

autores

Almeida, JS; Capela, EV; Loureiro, AM; Tavares, APM; Freire, MG

nossos autores

agradecimentos

This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was also financially supported by the project IL2BioPro (PTDC/BII-BBF/030840/2017), funded by FEDER, through COMPETE2020-Programa Operacional Competitividade e Internacionalizacao (POCI), and by national funds (OE), through FCT/MCTES. E.V. Capela acknowledges FCT for the PhD grant SFRH/BD/126202/2016. A.P.M. Tavares acknowledges FCT for the research contract CEECIND/2020/01867.

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