An Overview on the Recent Advances in Alternative Solvents as Stabilizers of Proteins and Enzymes

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abstract

Currently, the use of alternative solvents is increasing, namely ionic liquids (ILs) and deep eutectic solvents (DESs) in diverse fields of knowledge, such as biochemistry, chemistry, chemical engineering, biotechnology and biomedicine. Particularly, when compared to traditional solvents, these alternative solvents have great importance for biomolecules due to the enhanced solubility, structure stability and the biological activity of biomolecules, such as protein and enzymes. Thus, in this review article, the recent developments and efforts on the technological developments carried out with ILs and DESs for the stabilization and activation of proteins and enzymes are provided. The most studied IL- and DES-based formulations for proteins and enzymes are discussed and the molecular mechanisms and interactions related to the increased stability promoted by these alternative solvents are disclosed, while emphasizing their main advantages.

keywords

DEEP-EUTECTIC-SOLVENTS; AQUEOUS BIPHASIC SYSTEMS; IONIC LIQUIDS; THERMAL-STABILITY; SERUM-ALBUMIN; CHALLENGES; BINDING; ANTIBODIES; INSIGHT; LACCASE

subject category

Engineering

authors

Almeida, JS; Capela, EV; Loureiro, AM; Tavares, APM; Freire, MG

our authors

foto Ana Paula Mora Tavares

Ana Paula Mora Tavares

Assistant Researcher
foto Emanuel V. Capela

Emanuel V. Capela

Junior Researcher
foto Mara Guadalupe Freire Martins

Mara Guadalupe Freire Martins

Coordinating Researcher

Groups

G5 - Biomimetic, Biological and Living Materials

Projects

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

Towards the improvement of recombinant proteins bioprocessing using ionic liquids (IL2BioPro)

acknowledgements

This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was also financially supported by the project IL2BioPro (PTDC/BII-BBF/030840/2017), funded by FEDER, through COMPETE2020-Programa Operacional Competitividade e Internacionalizacao (POCI), and by national funds (OE), through FCT/MCTES. E.V. Capela acknowledges FCT for the PhD grant SFRH/BD/126202/2016. A.P.M. Tavares acknowledges FCT for the research contract CEECIND/2020/01867.

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Publication Details

type
Journal Article
year
2022
journal
CHEMENGINEERING
volume
6
article number
51
publisher
MDPI
issue
4
digital object identifier
10.3390/chemengineering6040051
web of science article identifier
WOS:000846967600001

Citations

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