resumo
We show the influence of two functional ions (Cu2+ and La3+), incorporated into a quaternary (Si, Ca, Na, P) sol-gel derived bioactive glass system, on its particle size, cytotoxicity, and bioactivity. By doping the parent glass with the two ions in singular or combined forms, 15 doped glasses were prepared by a rapid sol-gel technique. The influence of the combined doping on the particle size and cell viability was successfully evaluated by the aid of signal-to-noise-ratio (S/N), using Taguchi analysis. This allowed us to analyze the complex interplay of effects between these ions, and the marked differences in biocompatibility between the three cell types studied. Cu addition had a significant effect on reducing the glass particle size, while both increased density. Cell viability was significantly improved for some doping combinations, demonstrating that while combined Cu-La doping was beneficial for biocompatibility with lymphoblasts, individual high-Cu or low-La doping was better with fibroblasts, and either high-Cu or low-La doping, or certain combined Cu-La combinations, were the optimum for osteoblasts. However, the bioactivity of doped samples was generally similar to that of the parent glass, although both La, and particularly Cu, did appear to aid dissolution of ions when immersed in SBF, act as glass modifiers, and encourage HAp crystallization. The results reveal that potential synergistic benefits can be obtained by combining the effects on the mean particle size, density, cytotoxicity, and bioactivity of the glasses. The greatly improved biocompatibility of some of the doped glasses makes them promising candidates for biomedical applications.
palavras-chave
LANTHANUM OXIDE; GLASSES; SPECTROSCOPY; FTIR; SODIUM; SYSTEM
categoria
Engineering; Materials Science
autores
Ben-Arfa, BAE; Palama, IE; Salvado, IMM; Ferreira, JMF; Pullar, RC
nossos autores
Basam Abobaker Emhmed Ben-Arfa
Investigador
Isabel Margarida Miranda Salvado
Professor associado
José Maria da Fonte Ferreira
Professor(a) Jubilado(a)
Rob Pullar
ColaboradorGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
Projectos
agradecimentos
Fundacao para a Ciencia e a Tecnologia, Grant/Award Numbers: IF/00681/2015, PTDC/EPH-PAT/6281/2014, UID/CTM/50011/2019; Italian Association for Cancer Research (AIRC), Grant/Award Number: MFAG n. 16803; Tecnomed (FIRS project of nanotechnology, photonics and precision medicine)