Advanced Bottom-Up Engineering of Living Architectures

resumo

Bottom-up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom-up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular-rich structures or multicomponent cell-biomaterial synergies are described. An up-to-date critical overview of long-term existing and rapidly emerging technologies for integrative bottom-up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell-biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

palavras-chave

SHEAR-THINNING HYDROGELS; STIMULI-RESPONSIVE NANOCARRIERS; MAGNETIC CELLULAR SPHEROIDS; MESENCHYMAL STEM-CELLS; SURFACE SIALIC ACIDS; TISSUE CONSTRUCTS; EXTRACELLULAR-MATRIX; POLYMERIC NANOPARTICLES; SPATIOTEMPORAL CONTROL; BUILDING-BLOCKS

categoria

Chemistry; Science & Technology - Other Topics; Materials Science; Physics

autores

Gaspar, VM; Lavrador, P; Borges, J; Oliveira, MB; Mano, JF

Grupos

G5 - Materiais Biomiméticos, Biológicos e Vivos

Projectos

Bioengineered autonomous cell-biomaterials devices for generating humanised micro- tissues for regenerative medicine (ATLAS)

CICECO - Aveiro Institute of Materials (UID/CTM/50011/2019)

Produção de Plataformas Microcapsulares 3D+ para Avaliação Laboratorial em Larga Escala de Novas Terapias Combinatórias para o Cancro do Pâncreas (PANGEIA)

Cápsulas esféricas multicompartimentalizadas de alto rendimento para a transplantação de co-culturas multimodais de células estaminais derivadas do tecido adiposo e ilhotas pancreáticas. (TranSphera)

Desenvolvimento de hidrogeis de inspiracao marinha multifuncionais e de elevado desempenho para regeneracao de cartilagem, reticulados com ligacoes nao-covalentes e bio-instrutivas deslizantes. (MARGEL)

Dispositivos Supramoleculares Auto-Regulados baseados na Complementaridade dos Pares de Base do ADN: Uma Tecnologia Biomimética e com Reconheciemnto Molecular para Isolar Células Específicas para Aplicações Biomédicas (SUPRASORT)

agradecimentos

V.M.G. and P.L. contributed equally to this work. The authors would like to acknowledge the support of the European Research Council for project ATLAS, grant agreement ERC-2014-ADG-669858. This work was developed within the scope of the project CICECO - Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. This work was also supported by the Programa Operacional Competitividade e Internacionalizacao (POCI), in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the projects PANGEIA (PTDC/BTM-SAL/30503/2017), TranSphera (PTDC/BTM-ORG/30770/2017), Margel (PTDC/BTM-MAT/31498/2017), and by the Programa Operacional Regional do Centro - Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project SUPRASORT (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658). The PANGEIA project is acknowledged for the individual Junior Researcher contract of V.M.G. The authors also acknowledge financial support by FCT through a Ph.D. grant (SFRH/BD/141834/2018, P.L.) and through individual contracts (CEECIND/03202/2017, J.B.; CEECIND/03605/2017, M.O.).