One step selective partition of epsilon-polylysine present in broth cultures in ionic liquid-based aqueous biphasic systems

authors	Sequeira, RA; Sharma, M; Pereira, MM; Singh, N; Bhattacharya, S; Chudasama, NA; Prasad, K
nationality	International
journal	SEPARATION SCIENCE AND TECHNOLOGY
author keywords	Ionic liquid; epsilon-poly-L-lysine; polyamide; aqueous biphasic system; separation
keywords	2-PHASE SYSTEMS; PROTEIN SEPARATION; EXTRACTION; WATER
abstract	Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate epsilon-PL from culture broth. It was observed that the partition of epsilon-PL to the IL-rich phase was achieved, and with pure epsilon-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of epsilon-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of epsilon-PL from the culture broth.
publisher	TAYLOR & FRANCIS INC
issn	0149-6395
isbn	1520-5754
year published	2021
volume	56
issue	3
beginning page	631
ending page	639
digital object identifier (doi)	10.1080/01496395.2020.1728323
web of science category	9
subject category	Chemistry, Multidisciplinary; Engineering, Chemical
unique article identifier	WOS:000514054200001