One step selective partition of epsilon-polylysine present in broth cultures in ionic liquid-based aqueous biphasic systems


Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate epsilon-PL from culture broth. It was observed that the partition of epsilon-PL to the IL-rich phase was achieved, and with pure epsilon-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of epsilon-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of epsilon-PL from the culture broth.



subject category

Chemistry, Multidisciplinary; Engineering, Chemical


Sequeira, RA; Sharma, M; Pereira, MM; Singh, N; Bhattacharya, S; Chudasama, NA; Prasad, K

our authors


This work was supported by the Council of Scientific and Industrial Research, New Delhi.

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