resumo
Ionic liquids (ILs) have been applied in several fields in which enzymes and proteins play a noteworthy role, for instance in biorefinery, biotechnology, and pharmaceutical sciences, among others. Despite their use as solvents and co-solvents, their combination with materials for protein- and enzyme-based applications has raised significant attention in the past few years. Among them, significant advances were brought by supported ionic liquids (SILs), in which ILs are introduced to modify the surface and properties of materials, e.g., as ligands when covalently bond or when physiosorbed. SILs have been mainly investigated as alternative supports for enzymes in biocatalysis and as new supports in preparative liquid chromatography for the purification of high-value proteins and enzymes. In this manuscript, we provide an overview on the most relevant advances by using SILs as supports for enzymes and as purification platforms for a variety of proteins and enzymes. The interaction mechanisms occurring between proteins and SILs/ILs are highlighted, allowing the design of efficient processes involving SILs. The work developed is discussed in light of the respective development phase and innovation level of the applied technologies. Advantages and disadvantages are identified, as well as the missing links to pave their use in relevant applications.
palavras-chave
SOLID-PHASE EXTRACTION; BOVINE SERUM-ALBUMIN; MEDIATED EXTRACTION; SELECTIVE ISOLATION; IMMOBILIZED LIPASE; STABILITY; SEPARATION; CATALYSIS; PERFORMANCE; SURFACE
categoria
Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied
autores
Bento, RMF; Almeida, CAS; Neves, MC; Tavares, APM; Freire, MG
nossos autores
Ana Paula Mora Tavares
Investigador Auxiliar
Catarina Almeida
Estudante de doutoramento
Mara Guadalupe Freire Martins
Investigador Coordenador
Márcia Carvalho Neves
Investigador Auxiliar
Rui Miguel Farinha Bento
Bolseiro de investigaçãoProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Towards the use of IgY antibodies as alternative therapeutics (PureIgY)
agradecimentos
FundingThis work was developed within the scope of the projects CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, financed by national funds through FCT/MCTES and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. This work was funded by FEDER through COMPETE 2020-Programa Operacional Competitividade e Internacionalizacao (POCI), and by national funds (OE), through FCT/MCTES from the projects PTDC/BII-BBF/30840/2017 and POCI-01-0145-FEDER-031268. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 899921).