resumo
Scaffolds for bone tissue regeneration should provide the right cues for stem cell adhesion and proliferation, but also lead to their osteogenic differentiation. Hydrogels of modified platelet lysates (PLMA) show the proper mechanical stability for cell encapsulation and contain essential bioactive molecules required for cell maintenance. We prepared a novel PLMA-based nanocomposite for bone repair and regeneration capable of releasing biofactors to induce osteogenic differentiation. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were encapsulated in PLMA hydrogels containing bioactive mesoporous silica nanoparticles previously loaded with dexamethasone and functionalized with calcium and phosphate ions. After 21 d of culture, hBM-MSCs remained viable, presented a stretched morphology, and showed signs of osteogenic differentiation, namely the presence of significant amounts of alkaline phosphatase, bone morphogenic protein-2 and osteopontin, hydroxyapatite, and calcium nodules. Developed for the first time, PLMA/MSNCaPDex nanocomposites were able to guide the differentiation of hBM-MSCs without any other osteogenic supplementation.
categoria
Engineering, Biomedical; Materials Science, Biomaterials
autores
Tavares MT, Santos SC, Custodio CA, Farinha JPS, Baleizao C, Mano JF
nossos autores
Catarina de Almeida Custódio
Investigador Auxiliar
João Mano
Vice-Diretor
Sara Catarina Nunes da Silva Santos
Estudante de doutoramentoagradecimentos
This work was supported by Fundos Europeus Estruturais e de Investimento (FEEI), Programa Operacional Regional de Lisboa-FEDER (02/SAICT/2017), and national funds from Fundacao para a Ciencia e a Tecnologia (FCT-Portugal) and COMPETE (FEDER) within projects UIDB/00100/2020 and UIDP/00100/2020 (CQE), PTDC/CTM-POL/3698/2014, PTDC/CTM-CTM/32444/2017 (02/SAICT/2017/032444) and Margel (PTDC/BTM-MAT/31498/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. M. Tavares also thanks FCT for a PhD grant (FCT-PD/BD/114019/2015).