Platelet lysates-based hydrogels incorporating bioactive mesoporous silica nanoparticles for stem cell osteogenic differentiation
authors Tavares, MT; Santos, SC; Custodio, CA; Farinha, JPS; Baleizao, C; Mano, JF
nationality International
author keywords Nanocomposite; hBM-MSCs; Dexamethasone; Calcium and phosphate ions; Bone regeneration
abstract Scaffolds for bone tissue regeneration should provide the right cues for stem cell adhesion and proliferation, but also lead to their osteogenic differentiation. Hydrogels of modified platelet lysates (PLMA) show the proper mechanical stability for cell encapsulation and contain essential bioactive molecules required for cell maintenance. We prepared a novel PLMA-based nanocomposite for bone repair and regeneration capable of releasing biofactors to induce osteogenic differentiation. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were encapsulated in PLMA hydrogels containing bioactive mesoporous silica nanoparticles previously loaded with dexamethasone and functionalized with calcium and phosphate ions. After 21 d of culture, hBM-MSCs remained viable, presented a stretched morphology, and showed signs of osteogenic differentiation, namely the presence of significant amounts of alkaline phosphatase, bone morphogenic protein-2 and osteopontin, hydroxyapatite, and calcium nodules. Developed for the first time, PLMA/MSNCaPDex nanocomposites were able to guide the differentiation of hBM-MSCs without any other osteogenic supplementation.
publisher ELSEVIER
issn 2590-0064
year published 2021
volume 9
digital object identifier (doi) 10.1016/j.mtbio.2021.100096
web of science category 8
subject category Engineering, Biomedical; Materials Science, Biomaterials
unique article identifier WOS:000627056400007